e object is placedon the stage and lighted with the edge or face of the flame and then avery small diaphragm is put below the condenser. (If the Zeiss achro-matic condenser is used, the diaphragm of the Abbe illuminator servesfor this. If there is no pin-hole diaphragm one can be made of… … 42 LIGHTING AND FOCUSING. [CH. II. the small circle uncovered by the metal diaphragm. For the hole a fineneedle is best). If now the condenser is lowered or racked away fromthe objective the image of the diaphragm will appear. If the openingis not central it should be made so by using the centering…
Microscopy
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